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KMID : 0894520110150010031
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Development & Reproduction
2011 Volume.15 No. 1 p.31 ~ p.39
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Control of Ca²+ Influx by Ca²+ /Calmodulin Dependent Protein Kinase II in the Activation of Mouse Eggs
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Yoon Sook-Young
Kang Da-Won
Bae In-Ha
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Abstract
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Change in intracellular Ca²+ -concentration ([Ca²+]ᵢ) is an essential event for egg activation and further development. Ca²+ ion is originated from intracellular Ca²+ -store via inositol 1,4,5-triphosphate receptor and/or Ca²+ influx via Ca²+ channel. This study was performed to investigate whether changes in Ca²+ /calmodulin dependent protein kinase II (CaM KII) activity affect Ca²+ influx during artificial egg activation with ethanol using Ca²+ monitoring system and whole-cell patch clamp technique. Under Ca²+ ion-omitted condition, Ca²+ -oscillation was stopped within 30 min post microinjection of porcine sperm factor, and ethanol-induced Ca²+ increase was reduced. To investigate the role of CaM KII known as an integrator of Ca²+ - oscillation during mammalian egg fertilization, CaM KII activity was tested with a specific inhibitor KN-93. In the eggs treated with KN-93, ethanol failed to induce egg activation. In addition, KN-93 inhibited inward Ca²+ current ($I_{Ca}$ 수식 이미지) in a time-dependent manner in whole-cell configuration. Immunostaining data showed that the voltage-dependent Ca²+ channels were distributed along the plasma membrane of mouse egg and 2-cell embryo. From these results, we suggest that Ca²+ influx during fertilization might be controlled by CaM KII activity.
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KEYWORD
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Intracellular Ca²+-concentration([Ca²+]ᵢ), Ca²+/calmodulin dependent protein kinase II, Ca²+ influx, Egg activation
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